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Detection of chlamydia pneumoniae and Helicobacter pylori DNA in human atherosclerotic plaques by PCR
Scopus
Open Access Toplam 148 atıf DOI
Chlamydia pneumoniae and Helicobacter pylori can cause persistent infections of the respiratory and gastrointestinal tract, respectively. It has been suggested that persistent infection of arteries with these bacteria can contribute to the development of atherosclerosis. The aims of this study were to determine the presence of C. pneumoniae and H. pylori DNA in atherosclerotic plaque samples by PCR and to evaluate the correlation between clinical status and DNA positivity of these bacteria. Eighty-five consecutive patients (mean age, 59 ± 10; 75 male, 10 female) undergoing coronary artery bypass grafting, carotid endarterectomy, and surgery of the abdominal aorta for atherosclerotic obstructive lesions were included in the study. Forty-six endarterectomy specimens from the atherosclerotic lesions and 39 specimens from healthy regions of the ascending aorta, which were accepted as the control group, were excised. The presence of microorganism DNA in endarterectomy specimens was assessed by PCR. C. pneumoniae DNA was found in 12 (26%) of 46 endarterectomy specimens and none of the healthy vascular-wall specimens (P < 0.001), while H. pylori DNA was found in 17 (37%) of 46 endarterectomy specimens and none of the controls (P < 0.001). Either C. pneumoniae or H. pylori DNA was positive in 23 (50%) of 46 patients and none of the controls (P < 0.001). Six of the atherosclerotic lesions showed coexistence of both of the microorganism DNAs. The presence of C. pneumoniae and H. pylori DNA in a considerable number of atherosclerotic plaques but their absence in healthy vascular wall supports the idea that they may have a role in the development of atherosclerosis, especially in countries where infection is prevalent and where conventional risk factors fail to explain the high prevalence of atherosclerotic vascular disease.
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Investigation of the presence of Chlamydophila pneumoniae, Helicobacter pylori and cytomegalovirus in human atherosclerosis by molecular and serological methods
Scopus
Havuzumuzda 15 atıf almış
Persistant infections due to Chlamydophila pneumoniae, Helicobacter pylori, and Cytomegalovirus are suggested to contribute to the development of atherosclerosis. In this study, the presence of these agents was investigated by polymerase chain reaction (PCR) and serological tests in atherosclerotic plaques and non-atherosclerotic vessel samples. Thirty-three specimens from the atherosclerotic plaques (lesion group), 45 specimens from the healthy left internal mammarian arteries and ascending aortas of the atherosclerotic patients (non-lesion group), and 15 specimens from the aortas of non-atherosclerotic patients as determined by angiography (control group), were included to the study. Serum samples were also collected from all study subjects for the serological investigation. PCR and ELISA were used in order to determine the nucleic acid positivity and IgG titers, respectively. C.pneumoniae and H.pylori DNA were found in 6 (18.1%) and 14 (42.4%) of the lesion group, 2 (4.4%) and 9 (20%) of the non-lesion group, respectively. CMV DNA was not found in either groups. All of the PCR results of control group were negative. C.pneumoniae IgG was found positive in one case of the lesion group (3%) and three cases of the non-lesion group (6.7%). H.pylori IgG were positive in all the subjects except for three patients who were in the non-lesion group. The presence of C.pneumoniae and H.pylori DNA in a considerable number of lesion and non-lesion groups' specimens, but absence in control group specimens supports the hypothesis that these agents have an association with atherosclerosis.