Kurumun Atıf Alan Makalesi
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Anti-diabetic and anti-hyperlipidemic properties of Capparis spinosa L.: In vivo and in vitro evaluation of its nutraceutical potential
Scopus
Toplam 151 atıf DOI
In this study, the nutraceutical potential of Capparis spinosa L. for the treatment of hyperglycemic states has been thoroughly investigated. A series of in vivo and in vitro tests have been conducted on fresh leaf, buds and salty buds (24 h desalted) processed to dry powder. 60% MeOH/H2O extracts were obtained for HPLC analysis and for α-amylase and α-glucosidase inhibition tests. To estimate the in vivo anti-diabetic effect, dry powders of C. spinosa leaf and buds were orally administered to streptozocin-induced diabetic rats over a period of 28 days. At the end of the experiment, animals were sacrificed, blood taken for assessment of lipid profile and liver/kidney biochemistry while section of the pancreas, liver and kidneys were processed for general histology. Results showed that the regular administration of C. spinosa leaf or buds normalized all the biochemical parameters and reversed the liver/kidney injury with variable degrees of organ protection.
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Atıf Yapan Yayın
In vitro antioxidant and enzyme inhibitory studies, computational analysis and chemodiversity of an emergency food plant Caralluma edulis (Edgew.) Benth. ex Hook.f: A multifunctional approach to provide new ingredients for nutraceuticals and functional foods
Scopus
Havuzumuzda 14 atıf almış
Caralluma edulis is a leafless succulent herb native to India and Pakistan, where it is used as emergency food and as potential herbal medicine. It was investigated for its bioactive chemicals and further medicinal properties. The objective of the current study was to accomplish the chemical and biological characterization of C. edulis. Chemical profiling was done through estimation of total bioactive contents and UHPLMS analysis. Biological screening was achieved through six different antioxidant and enzyme inhibition assays. Estimation of its phenolic and flavonoid contents revealed that ethyl acetate fraction (Ce-E) contains 14.15 mg GAE/g extract and 26.13 mg RE/g extract of phenolics and flavonoids, respectively, followed by the methanolic (Ce-M) and the water (Ce-W) soluble fractions. In free radical inhibitory assays, Ce-E fraction also exhibited highest activity (DPPH: 19.72 mg TE/g extract, ABTS: 48.66 mg TE/g extract) followed by Ce-M, whereas, in CUPRAC and FRAP assays, all the extracts, except water fraction, exhibited nearly equal potential in the range of 53.85–57.96 and 25.42–32.12 mg TE/g extract, respectively. In metal chelating antioxidant assay, only Ce-M and Ce-W fractions displayed considerable activities (25.88 and 23.55 mg EDTAE/g extract respectively), whereas, Ce-H exhibited the highest activity in phosphomolybdenum assay (1.03 mmolTE/g extract), against BChE (6.43 mg GALAE/g extract), α-glucosidase and α-amylase (6.98 and 0.47 mmol ACAE/g, respectively) and tyrosinase (64.46 mg KAE/g extract) enzymes. Ce-M and Ce-E also exhibited significant activity against tyrosinase (59.85 and 58.40 mg KAE/g extract, respectively). Crude methanolic extract (Ce-M) was analyzed to unveil its secondary metabolic picture; the UHPLCMS analysis discloses 105 compounds as phenolic acids, flavonoids, steroids and their glycosides, which makes the C. edulis extract a strong candidate for nutraceutical and functional food ingredient. Some of the metabolites were docked against all the tested enzymes to predict mode of actions and to substantiate the pharmaceutical nature of C. edulis.
Atıf Yapan Makale Bilgileri
Kurumlar (8)
King Khalid University
Abha, Saudi Arabia
Ministry of Health Saudi Arabia
Riyadh, Saudi Arabia
Selçuk Üniversitesi
Selçuklu, Turkey
The Government Sadiq College Women University Bahawalpur
Bahawalpur, Pakistan
The Islamia University of Bahawalpur
Bahawalpur, Pakistan
University of Education
Lahore, Pakistan
University of G. d'Annunzio Chieti and Pescara
Chieti, Italy
University of Karachi
Karachi, Pakistan