Kurumun Atıf Alan Makalesi
Atıf Alan Yayın
Photothermal Antibacterial and Antibiofilm Activity of Black Phosphorus/Gold Nanocomposites against Pathogenic Bacteria
Scopus
Toplam 165 atıf DOI
Black phosphorus (BP) as a layered two-dimensional (2D) semiconductor material with a tunable band gap has attracted growing attention for promising applications in diverse fields including biotechnology owing to its excellent physical and chemical properties. In this study, BP crystals were synthesized using a chemical vapor transport method and exfoliated into BP nanosheets in deoxygenated water or hexane. Next, monodisperse Au nanoparticles that were synthesized using a surfactant-assisted chemical reduction method were assembled on exfoliated BP nanosheets hexane to yield BP/Au nanocomposites. The photothermal antibacterial and antibiofilm activities of BP nanosheets and BP/Au nanocomposites were investigated against Enterococcus faecalis, a pathogenic biofilm-forming bacterium, by studying the photothermal effect and bacterial growth curve and using colony counting and live/dead fluorescence staining methods under near-infrared (NIR) light irradiation. Thanks to the higher photothermal conversion efficiency of BP/Au nanocomposites than that of bare BP nanosheets under NIR light irradiation, they destructed the bacterial cell membrane more efficiently than bare BP with the biofilm inhibition rate of 58%. It should be noted that this is the first study on the antibacterial and antibiofilm activity of BP/Au nanocomposites via a photothermal process under NIR light irradiation. This work shows the potential of BP/Au nanocomposites in fighting against pathogenic bacteria and paves the way for the exploration of antibacterial platforms based on the biocompatible 2D semiconductor BP.
Atıf Kaynağı
Atıf Yapan Yayın
Superb Photo-Antibacterial/Antibiofilm Activities of BP/WS2 and BP/MoS2 Nanocomposites under Near-Infrared Irradiation
Scopus
Havuzumuzda 9 atıf almış
The development of multifunctional antibacterial materials based on semiconductor materials has become the focus of treatment in the field of antibiotic-resistant bacteria. Here, for the first time, the superb photocatalytic/photothermal antibacterial activity of BP/MoS2 and BP/WS2 nanocomposites against both E. coli and S. aureus under near-infrared (NIR) or light-emitting diode (LED) illumination was demonstrated. Characterization studies using advanced instrumental techniques confirmed the successful formation of BP/WS2 and BP/MoS2 S-scheme heterojunctions with distinct structural, morphological, and compositional features. Moreover, it was verified that the enhanced photo-antibacterial activity of both heterojunctions compared to their pristine analogs was demonstrated to be due to the synergistic interactions at the atomic level elucidated by Mo/W-S-P via a sulfur atom bridge in X-ray photoelectron spectroscopy analysis, which improves the charge flow and enhances the photocatalytic performance. Optical density measurements were performed to obtain bacterial growth over 4 h, where BP/WS2 (≅88%) and BP/MoS2 (≅83%) showed higher NIR light-driven antibacterial activity compared to the pristine analogs (BP nanosheets, WS2 or MoS2 nanostructures), which is attributed to the S-scheme heterojunctions formed between BP and MS2 that enhance the production of ROS by promoting the use of light-induced carriers. The photo-antibacterial activities of BP/WS2 and BP/MoS2 heterojunctions reached 93% and 98%, respectively. The bacterial mechanical rupture effect of BP/WS2 and BP/MoS2 heterojunctions was monitored using SEM and E. coli was found to be more resistant to damage than S. aureus. The ability of BP/WS2 and BP/MoS2 to generate reactive oxygen species (ROS) was better than that of pristine BP nanosheets, as demonstrated by a glutathione (GSH) oxidation assay. The photothermal activities of the nanocomposites were investigated to explain the photoinduced antibacterial mechanism. In addition, the photo-antibiofilm activities of BP/WS2 and BP/MoS2 heterojunctions were also investigated and the biofilm structure of S. aureus was almost completely eradicated under LED light irradiation.