Kurumun Atıf Alan Makalesi
Atıf Alan Yayın
Photothermal Antibacterial and Antibiofilm Activity of Black Phosphorus/Gold Nanocomposites against Pathogenic Bacteria
Scopus
Toplam 165 atıf DOI
Black phosphorus (BP) as a layered two-dimensional (2D) semiconductor material with a tunable band gap has attracted growing attention for promising applications in diverse fields including biotechnology owing to its excellent physical and chemical properties. In this study, BP crystals were synthesized using a chemical vapor transport method and exfoliated into BP nanosheets in deoxygenated water or hexane. Next, monodisperse Au nanoparticles that were synthesized using a surfactant-assisted chemical reduction method were assembled on exfoliated BP nanosheets hexane to yield BP/Au nanocomposites. The photothermal antibacterial and antibiofilm activities of BP nanosheets and BP/Au nanocomposites were investigated against Enterococcus faecalis, a pathogenic biofilm-forming bacterium, by studying the photothermal effect and bacterial growth curve and using colony counting and live/dead fluorescence staining methods under near-infrared (NIR) light irradiation. Thanks to the higher photothermal conversion efficiency of BP/Au nanocomposites than that of bare BP nanosheets under NIR light irradiation, they destructed the bacterial cell membrane more efficiently than bare BP with the biofilm inhibition rate of 58%. It should be noted that this is the first study on the antibacterial and antibiofilm activity of BP/Au nanocomposites via a photothermal process under NIR light irradiation. This work shows the potential of BP/Au nanocomposites in fighting against pathogenic bacteria and paves the way for the exploration of antibacterial platforms based on the biocompatible 2D semiconductor BP.
Atıf Kaynağı
Atıf Yapan Yayın
Bismuthene nanosheets as a photodynamic and photothermal antibacterial agent under NIR light illumination
Scopus
Havuzumuzda 4 atıf almış
Bacterial infections remain a significant public health burden due to the emergence of antibiotic resistance and their non-specific cytotoxic effects, leading to the search for novel antibacterial agents. Two-dimensional (2D) pnictogens, which stand out with their advantegeous properties such as large surface areas, compatibility with biological systems, and permeability across biological membranes, have emerged as potential materials in the fight against bacterial infections. By considering all these advantages, here for the first time, the antibacterial activity of 2D bismuth (Bismuthene, Biene) on Gram-negative Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa), Gram-positive Staphylococcus aureus (S. aureus) and Methicillin-Resistant Staphylococcus aureus (MRSA) were examined under NIR light illumination. A growth curve analysis was conducted with a concentration of 256 μg*mL−1 of exfoliated Biene nanosheets to assess the inhibition effect and corresponding antibacterial effect (%) against each bacterial strain. The photodynamic theraphy (PDT) and photothermal therapy (PTT)-mediated antibacterial mechanisms were explored by analyzing the generation of reactive oxygen species (ROS) via Glutathione (GSH) oxidation assay while a photothermal camera monitored temperature dynamic changes during irradiation. The high specific surface area-dependent membrane damage ability of Biene and morphological changes of the bacteria were visualized by field emission scanning electron microscope (FESEM). The exciting growth inhibition activity of Biene nanosheets for all bacterial strains was increased during irradiation, and breathtakingly the inhibition rate reached up to ≥ 99.1 % for P. aeruginosa, S. aureus, and MRSA. Besides, S. aureus and MRSA are more susceptible to Biene than E. coli and P. aeruginosa.
Atıf Yapan Makale Bilgileri
Kurumlar (2)
Koç University
Istanbul, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey