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Total phenolics, total carotenoids, individual phenolics and antioxidant activity of ginger (Zingiber officinale) rhizome as affected by drying methods
Scopus
Toplam 178 atıf DOI
This study was conducted to evaluate total phenolic content, antioxidant activity, total carotenoids and phenolic compounds of ginger (Zingiber officinale) rhizomes dried using different drying methods (oven, microwave, freeze and room-air drying). Freeze-dried ginger rhizomes exhibited significantly (p < 0.05) higher total phenolics (931.94 mgGAE/100 g), antioxidant activity (82.00%) and total carotenoids (13.17 μg/g) than those dried using other techniques. Oven-dried ginger showed higher values for all individual phenolic compounds detected in this study as compared to those dried using other methods. The dominant phenolic compounds of dried ginger included (+)-catechin (250.02 mg/100 g), gallic acid (197.03 mg/100 g) and 3, 4-dihydroxybenzoic acid (116.07 mg/100 g). Drying of ginger rhizome using oven and freeze-drying methods my result in improved contents of bioactive compounds and higher antioxidant activity. Correlation analyses for biochemical composition of ginger rhizomes showed that total phenolics were positively correlated with antioxidant activity (r2 = 0.973, p < 0.001).
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Screening of bioactive metabolites and biological activities of calli, shoots, and seedlings of mertensia maritima (L.) gray
Scopus
Havuzumuzda Open Access 8 atıf almış
Mertensia maritima (L.) Gray is threatened with extinction owing to climate change, poor seed germination, and ocean warming. In vitro explant-culture is used for ex situ preservation and plantlet massive production. In vitro cell and organ cultures serve as an alternative plant material source to investigate the biological activities and phytochemical profiles of rare plants. We aimed to develop an efficient callus and shoot production protocol and investigate bioactive metabolites, antioxidants, and enzyme inhibitory potential of M. maritima calli, shoots, and in vivo seedlings. The effects of combinations of different plant growth regulators, 6-BA (N6-benzyladenine), 6-KN (Kinetin), TDZ (Thidiazuron), and NAA (1-Naphthylacetic acid), in MS (Murashige and Skoog) nutrient medium were studied. The highest callus proliferation was obtained after 5-week cultivation over a 16-h photoperiod on growth medium MS enriched with 4 µM each of 6-BA and NAA. The medium with 2 µM 6-BA and 4 µM 6-KN had the best shoot induction rate (91.1%) with a mean of 13.4 shoots. The combination of two cytokinins (6-BA and 6-KN) was found to be effective in M. maritima shoot regeneration. The rooting frequency was 100% in12 MS with Indole-3-butyric acid (IBA 2 µM). The number of detected compounds and chemical composition in the M. maritima shoots and seedlings extracts were similar. The total amount of phenolics in the shoots was 216.4% and 369.5% higher than in seedlings and calli, respectively. The total amount of flavonoids in the shoots was 241.1% and 429.3% higher than in seedlings and calli, respectively. The best antioxidant activity was obtained in the shoots, followed by seedlings and calli. However, the order was seedlings > calli > shoots regarding metal chelating ability. The strongest acetylcholinesterase inhibition properties were obtained in the calli, followed by seedlings and shoots. However, the tested samples can be ranked as seedlings > shoots > calli in butylcholinestrase inhibition assay. This study is the first report on the enzyme inhibitory effects of M. maritima extracts, providing valuable contributions to the scientific community.
Atıf Yapan Makale Bilgileri
Kurumlar (6)
Duy Tan University
Da Nang, Viet Nam
Konkuk University
Seoul, South Korea
North-West University
Potchefstroom, South Africa
Sejong University
Seoul, South Korea
Selçuk Üniversitesi
Selçuklu, Turkey
University of Nyíregyháza
Nyíregyháza, Hungary