Kurumun Atıf Alan Makalesi
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The influence of cysteine and taurine on microscopic-oxidative stress parameters and fertilizing ability of bull semen following cryopreservation
Scopus
Toplam 192 atıf DOI
Oxidative stress significantly damages sperm functions such as motility, functional integrity, endogenous antioxidant enzyme activities and fertility due to lipid peroxidation induced by reactive oxygen species (ROS). The aim of this study was to determine the effects of antioxidants such as taurine and cysteine in Bioxcell® extender on standard semen parameters, fertilizing ability, lipid peroxidation (LPO) and antioxidant activities comprising reduced glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD) after the cryopreservation/thawing of bull semen. Nine ejaculates for each bull were included in the study. Three groups, namely taurine (2 mM), cysteine (2 mM), and control, were designed to analyze the antioxidants in Bioxcell®. Insemination doses were processed so that each 0.25-ml straw contained 15 × 106 sperm. The addition of cysteine led to higher motility, compared to the other groups (P < 0.001). Cysteine showed a greater protective effect on the percentages of acrosome damage and total abnormalities in comparison to the other groups (P < 0.001). No significant differences were observed in hypo-osmotic swelling test (HOST), following supplementation with antioxidants during the freeze-thawing process. No significant difference was observed in non-return rates among groups. In biochemical assays, the additives did not show effectiveness on the elimination of malondialdehyde (MDA) formation and maintenance of GSH and GSH-Px activities, when compared to controls. CAT activity (35.1 ± 8.1 kU/g) was demonstrated to be significantly higher upon the addition of 2 mM taurine (P < 0.001), while the level of MDA increased, indicating oxidative stress in this group. SOD activity (21.4 ± 2.9 U/g protein) was significantly elevated in the group with cysteine, compared to the other groups (P < 0.001). Crown Copyright © 2008.
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Atıf Yapan Yayın
The effect of raffinose and methionine on frozen/thawed Angora buck (Capra hircus ancryrensis) semen quality, lipid peroxidation and antioxidant enzyme activities
Scopus
Havuzumuzda 75 atıf almış
The aim of the present study was to determine the effects of different doses of raffinose and methionine on post-thawed semen quality, lipid peroxidation and antioxidant enzyme activities of Angora buck (Capra hircus ancryrensis) sperm following cryopreservation.Ejaculates collected from three Angora bucks were evaluated and pooled at 37 °C. Semen samples, which were diluted with a Tris-based extender containing the additives raffinose (2.5, 5, 10. mM) and methionine (2.5, 5, 10. mM) and an extender containing no antioxidants (control), were cooled to 5 °C and frozen in 0.25. ml French straws. Frozen straws were thawed individually at 37 °C for 20. s in a water bath for evaluation. The freezing extender supplemented with 2.5 and 5. mM methionine led to higher percentages of CASA motility (63.6 ± 7.0; 63.4 ± 3.1%, respectively), in comparison to the controls (P< 0.01) following the freeze-thawing process. The addition of antioxidants did not provide any significant effect on the percentages of post-thaw subjective and CASA progressive motilities as well as sperm motion characteristics (VSL and VCL), compared to the control groups (P> 0.05). The freezing extender with raffinose (5 and 10. mM) and methionine at three different doses (2.5, 5 and 10. mM) led to lower percentages of acrosome abnormalities, in comparison to the controls (P< 0.001). In the comet test, raffinose (5 and 10. mM) and methionine (10. mM) gave scores lower than those of the controls, and thereby reduced DNA damage (P< 0.05). Malondialdehyde formation was found to be lower (1.8 ± 0.1. nmol/L) in the group of 5. mM raffinose, compared to the controls following the freeze-thawing process (P< 0.01). The additives did not show any effectiveness on the maintenance of SOD, GSH-PX and GSH activities, when compared to the controls (P> 0.05). In conclusion, methionine and raffinose play a cryoprotective role against sperm CASA motility, acrosome abnormality and DNA damage. Raffinose 5. mM exhibited antioxidative properties, decreasing MDA levels. Further studies are required to obtain more concrete results on the characterization of microscopic parameters and antioxidant activities in cryopreserved goat sperm with different additives. © 2010.
Atıf Yapan Makale Bilgileri
Kurumlar (6)
Afyon Kocatepe Üniversitesi
Afyonkarahisar, Turkey
Ankara Üniversitesi
Ankara, Turkey
Erciyes Üniversitesi
Kayseri, Turkey
Firat Üniversitesi
Elazig, Turkey
Mustafa Kemal Üniversitesi
Antakya, Turkey
T.C. Tarim ve Köyişleri Bakanliği
Antakya, Turkey