Kurumun Atıf Alan Makalesi
Atıf Alan Yayın
Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen
Scopus
Open Access Toplam 198 atıf DOI
Free radicals are known to be involved in lipid peroxidation as well as DNA and sperm membrane damages that may lead to decreased sperm motility or cell death. The balance between free radical production and their detoxification may be an important factor in sperm survival and function before, during and after cryopreservation. The aim of this study was to determine the effects of the addition of the antioxidants of oxidized glutathione (GSSG), bovine serum albumin (BSA), cysteine and lycopene to freezing media on the post-thawing sperm characteristics, including motility, morphology, acrosome integrity, viability and membrane integrity. A total number of 42 ejaculates were collected using the artificial vagina from 4 Akkaraman rams and 10 replicates of the ejaculates were diluted with a Tris-based extender containing additives and no additives as control. GSSG (5 mM), BSA (20 mg/ml), cysteine (10 mM) and lycopene (800 μg) showed more positive effects than other concentrations of the supplements and controls in protecting sperm characteristics after the freezing-thawing process (P < 0.001). Many aspects of sperm protection, e.g. sperm motility, viability and membrane stabilisation of the sperm cells during relative cryopreservation, are the key factors in determining the preservation of sperm function. The results of this study provide a new approach to the cryopreservation of sperm from rams and related breeds, and thereby contribute to the improvement of these breeds for the world sheep industry.
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Atıf Yapan Yayın
The effect of cysteine and glutathione on sperm and oxidative stress parameters of post-thawed bull semen
Scopus
Havuzumuzda 121 atıf almış
The aim of this study was to determine the effects of antioxidants such as reduced glutathione (GSH) and cysteine in Laiciphose® extender on semen parameters, fertilizing ability, lipid peroxidation (LPO) level and glutathione peroxidise (GPx) activity of post-thawed bull semen. Totally 54 ejaculates of three bulls were used in the study. Five groups, namely; GSH (0.5 and 2mM), cysteine (5 and 10mM) and control group, were conducted to test the antioxidants in Laiciphose®. Insemination doses were processed that each 0.25-mL straw contained 15×106 sperm. The addition of antioxidants did not present any significant effect on the percentages of post-thaw sperm morphology (acrosome and total abnormalities), subjective, CASA and progressive motilities, as well as sperm motility characteristics (VAP, VSL, VCL, LIN and ALH), compared to the control groups (P>0.05). GSH 0.5mM (55.5±7.38%) and cysteine 10mM (48±5.65%) led to lower rates of DNA damage, compared to control (P<0.05). As regards to MDA level, cysteine at 10mM dose gave the highest level (4.99±0.44nmol/L) (P<0.001). GPx activity was demonstrated to be higher level upon the addition of 5mM cysteine when compared to the other groups (P<0.05). With respect to fertility results based on 60-day non-returns, the supplementation of antioxidants did not present significant differences (P>0.05). The results of this study may provide an useful information for the future studies in this area. So, further studies could be suggested to achieve better information in terms of the DNA damage and fertilizing capacity of bull sperm frozen with effective antioxidants. © 2010.
Atıf Yapan Makale Bilgileri
Kurumlar (5)
Afyon Kocatepe Üniversitesi
Afyonkarahisar, Turkey
Erciyes Üniversitesi
Kayseri, Turkey
Gülhane Eğitim ve Araştırma Hastanesi
Ankara, Turkey
T.C. Tarim ve Köyişleri Bakanliği
Antakya, Turkey
T.C. Tarim ve Köyisleri Bakanligi, Turkey
Ankara, Turkey