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Atıf Alan Yayın
The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen. Microscopic and oxidative stress parameters after freeze-thawing process
Scopus
Toplam 341 atıf DOI
There is a lack of information regarding lipid peroxidation and antioxidant capacity in cryopreserved ram semen, and cryopreservation is associated with the production of reactive oxygen species (ROS) which lead to lipid peroxidation (LPO) of sperm membranes, resulting in a loss of motility, viability and fertility of sperm. The aim of this study was to determine the influence of certain additives and their different doses on standard semen parameters, lipid peroxidation and antioxidant activities after the cryopreservation/thawing of ram semen. Ejaculates collected from four Akkaraman rams, a native breed of sheep, were evaluated and pooled at 33 °C. Semen samples which were diluted with a Tris-based extender containing additives including trehalose (50, 100 mM), taurine (25, 50 mM), cysteamine (5, 10 mM), and hyaluronan (0.5, 1 mg/ml), and an extender containing no additives (control) were cooled to 5 °C and frozen in 0.25 ml French straws, being stored in liquid nitrogen. Frozen straws were thawed individually at 37 °C for 20 s in a water bath for evaluation. The use of a Tris-based extender supplemented with 50 mM trehalose, 25 mM taurine, and 5 and 10 mM cysteamine led to higher percentages of post-thaw motility, in comparison to the control group (P < 0.01). No significant differences were observed in the percentages of acrosome and total abnormalities, and the hypoosmotic swelling test upon the supplementation of the freezing extender with antioxidants after the thawing of semen. In biochemical assays, the addition of antioxidants did not cause significant differences in levels of malondialdehyde (MDA), glutathione (GSH), and glutathione peroxidase (GSH-Px), after thawing, when compared to groups with no additives. In this study, catalase (CAT) activities were higher in the group that was applied 25 mM taurine as an antioxidant, than in all of the other groups (P < 0.001). Compared to the controls, antioxidant treatment with 100 mM trehalose, 50 mM taurine, 5 mM cysteamine and 0.5 mg/ml hyaluronan, significantly elevated vitamin E (vit E) levels in samples (P < 0.001).
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Atıf Yapan Yayın
Effects of trehalose, melatonin and lipid mixtures on bull sperm parameters and gene expressions during cryopreservation
Scopus
Havuzumuzda Open Access
The objective of this study was to investigate the effects of trehalose and different doses of melatonin and lipid mixtures on the quality parameters of post-thaw bull sperm during cryopreservation. The ejaculates of three mature bulls were pooled and divided into ten equal aliquots. These aliquots were diluted with a Tris-based extender, which was supplemented with either 5% glycerol (G5) or 3% glycerol combined with 60 mM trehalose (G3T), alongside different doses of melatonin and lipid mixtures. Ten experimental groups were established as follows: G5, G5+0.25 mM melatonin (G5M0.25), G5+0.75 mM melatonin (G5M0.75), G5+ 1.25 μl/ml lipid mixtures (G5L1.25), G5+3.75 μl/ml lipid mixtures (G5L3.75), G3T, G3T+0.25 mM melatonin (G3TM0.25), G3T+0.75 mM melatonin (G3TM0.75), G3T+1.25 μl/ml lipid mixtures (G3TL1.25), and G3T+3.75 μl/ml lipid mixtures (G3TL3.75). No significant interaction was detected between any of the groups containing the G5 and G3T extenders, and all groups were found to be similar for sperm motility and flow cytometry analysis results (p > 0.05). The G5M0.25 and G5L1.25 groups had a higher recovery of post-thaw motility compared to the other groups containing 5% glycerol (p = 0.0004). In terms of motility rates, groups G3TM0.25 and G3TL1.25 displayed a higher level of protection compared to the other groups, and this protection was significantly greater than that determined in groups G3TM0.75 and G3TL3.75 (p = 0.001). The expression of the GFPT1, PFKP, FBF2, HK1, and ALDH2 genes was found to be significantly increased in the G5 and G3T groups containing both doses of lipid mixtures (L1.25 and L3.75) compared to the groups without additives (G5 and G3T) (p < 0.001-0.0001). However, the expression of the SORD gene was found to be increased only in the G3TL1.25 and G3TL3.75 groups compared to group G3T (p < 0.01-0.001). GFPT1 and FBF2 gene expressions were significantly increased in the G5M0.75 group compared to group G5 (p < 0.05). The G3T groups containing both doses of melatonin were found to display increased GFPT1 (p < 0.01) and PFKP (p < 0.05-0.01) expression levels compared to group G3T. It was determined that the addition of lipid mixtures at both doses to both G5 and G3T resulted in a significant transcriptional increase in all of the genes studied (except for SORD gene expression in the G5L1.25 and G5L3.75 groups), compared to the lipid mixtures-free groups (p < 0.05-0.001). Based on the evaluation of all results, G3T can be used as a substitute for G5 to reduce glycerol toxicity.
Atıf Yapan Makale Bilgileri
Kurumlar (3)
Burdur Mehmet Akif Ersoy Üniversitesi
Burdur, Turkey
Necmettin Erbakan Üniversitesi
Meram, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey