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A comprehensive study on phytochemical characterization of Haplophyllum myrtifolium Boiss. endemic to Turkey and its inhibitory potential against key enzymes involved in Alzheimer, skin diseases and type II diabetes
Scopus
Toplam 222 atıf DOI
The interest of medicinal plants as therapeutic agents against diseases such as Alzheimer, skin disorders and diabetes mellitus is growing. In order to investigate the influence of Haplophyllum myrtifolium, four solvent extracts (petroleum ether, ethyl acetate, methanol and water) were screened for antioxidant potentials, anti-cholinesterase, anti-tyrosinase, anti-amylase and anti-glycosidase activity. Antioxidant effects were elucidated by different assays including free radical scavenging (ABTS, DPPH and NO assay), reducing power (FRAP and CUPRAC), phosphomolybdenum, β-carotene/linoleic acid system and metal chelating. Total phenolic, flavonoid, flavanol, tannin and saponin contents in these extracts were also calculated. Generally, ethyl acetate extract showed the strongest antioxidant activity in these extracts. This activity may be related to a good total phenolic content. The total phenolic contents of these extracts ranged from 32.32 to 52.50. mg GAEs/g extract. Except for water extract, all extracts showed good inhibitory activities on cholinesterase, tyrosinase, amylase and glycosidase. Apolar extracts (petroleum ether and ethyl acetate) were more potent as enzyme inhibitors in comparison to polar extracts (methanol and water). These findings reveal that H. myrtifolium could be used a source of natural compounds for the management of oxidative damage, Alzheimer, skin disorders and diabetes mellitus. © 2014 Elsevier B.V.
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DNA protection, antioxidant, antibacterial and enzyme inhibition activities of heartwood and sapwood extracts from juniper and olive woods
Scopus
Havuzumuzda 17 atıf almış
In this study, DNA protective, antioxidant, antibacterial and enzyme inhibiting properties of methanol extracts obtained from juniper and olive heartwood and sapwood were determined. These extracts were tested by five antioxidant methods (DPPH scavenging, FRAP, CUPRAC, metal chelating and phosphomolybdenum). Generally, heartwood extracts of both species are more efficient for DPPH radical scavenging activity, cupric ion reducing activity, ferric reducing antioxidant power and metal chelating activity than sapwood extracts. When compared to heartwood extracts, sapwood extracts have larger inhibition zone in disk diffusion test. In addition, all extracts showed high antibacterial activity against Staphylococcus aureus. DNA protection of both extracts had a capacity to inhibit the DNA damage arisen from Fenton's reagent. The highest DNA protective activity was observed in juniper sapwood extract with 84%. Furthermore, other extracts also indicated more than 60% of DNA protective activity. Olive wood extracts displayed the strongest enzyme inhibition activities against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Although juniper heartwood extracts showed highest anti-amylase, anti-glucosidase and anti-tyrosinase effects, they had no ability for inhibition BChE. The methanol extracts of olive samples demonstrated the most antioxidant activity (DPPH, CUPRAC and FRAP). In addition, juniper samples showed the highest anti-amylase, anti-tyrosinase, metal chelating and DNA protective activity. According to these results, the extracts of juniper and olive wood can be considered as a source of natural bio active agents for dietary, pharmacological and medicinal applications. This research will also serve as a base for future studies about biological activities of wood extracts.
Atıf Yapan Makale Bilgileri
Kurumlar (2)
Kastamonu University
Kastamonu, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey