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A comprehensive study on phytochemical characterization of Haplophyllum myrtifolium Boiss. endemic to Turkey and its inhibitory potential against key enzymes involved in Alzheimer, skin diseases and type II diabetes
Scopus
Toplam 222 atıf DOI
The interest of medicinal plants as therapeutic agents against diseases such as Alzheimer, skin disorders and diabetes mellitus is growing. In order to investigate the influence of Haplophyllum myrtifolium, four solvent extracts (petroleum ether, ethyl acetate, methanol and water) were screened for antioxidant potentials, anti-cholinesterase, anti-tyrosinase, anti-amylase and anti-glycosidase activity. Antioxidant effects were elucidated by different assays including free radical scavenging (ABTS, DPPH and NO assay), reducing power (FRAP and CUPRAC), phosphomolybdenum, β-carotene/linoleic acid system and metal chelating. Total phenolic, flavonoid, flavanol, tannin and saponin contents in these extracts were also calculated. Generally, ethyl acetate extract showed the strongest antioxidant activity in these extracts. This activity may be related to a good total phenolic content. The total phenolic contents of these extracts ranged from 32.32 to 52.50. mg GAEs/g extract. Except for water extract, all extracts showed good inhibitory activities on cholinesterase, tyrosinase, amylase and glycosidase. Apolar extracts (petroleum ether and ethyl acetate) were more potent as enzyme inhibitors in comparison to polar extracts (methanol and water). These findings reveal that H. myrtifolium could be used a source of natural compounds for the management of oxidative damage, Alzheimer, skin disorders and diabetes mellitus. © 2014 Elsevier B.V.
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Chemical characterization with in vitro biological activities of Gypsophila species
Scopus
Havuzumuzda 31 atıf almış
Methanol-aqueous extracts from the aerial parts of Gypsophila glomerata (GGE), G. trichotoma (GTE) and G. perfoliata (GPE) were investigated for antioxidant potential using different in vitro models, as well as for phenolic and flavonoid contents. The possible anti-cholinesterase, anti-tyrosinase, anti-amylase and anti-glucosidase activities were also tested. The flavonoid variability was analyzed using ultra high-performance liquid chromatography (UHPLC) coupled with hybrid quadrupole-Orbitrap high resolution mass spectrometry (HRMS). Eleven C-glycosyl flavones and 4 O-glycosyl flavonoids, including 2”-O-pentosyl-6-C-hexosyl-apigenin/methylluteolin, as well as their mono(di)-acetyl derivatives were found in GGE. Both GGE and GTE shared 2”-pentosyl-6-C-hexosyl-luteolin together with the common saponarin, homoorientin, orientin, isovitexin and vitexin, while di C-glycosyl flavones were evidenced only in GPE. The highest radical scavenging in both ABTS and DPPH assays was noted in GPE, as well as ferric and cupric reducing abilities. However, GTE had the strongest metal chelating activity (17.44 ± 0.51 mg EDTAE/g extract). GPE and GGE were more potent as acetylcholinesterases inhibitors witnessed by 2.09 ± 0.02 mg GALAE/g extract and 1.59 ± 0.09 mgGALAE/g extract, respectively. All flavonoids were found in G. glomerata for the first time. Therefore, further isolation and structural elucidation of newly described acetylated flavonoids are needed in order to determine their relevance in the beneficial properties of the plant.
Atıf Yapan Makale Bilgileri
Kurumlar (2)
Medical University of Sofia
Sofia, Bulgaria
Selçuk Üniversitesi
Selçuklu, Turkey