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Screening the in vitro antioxidant, antimicrobial, anticholinesterase, antidiabetic activities of endemic Achillea cucullata (Asteraceae) ethanol extract
Scopus
Open Access Toplam 223 atıf DOI
The Achillea genus belongs to the Asteraceae family, which is mostly found in the northern hemisphere and is comprised of 115 species in the world. In Turkish flora, there are 52 species and 58 taxa, among them half of which are recorded as endemic. To the best of our knowledge, there has been no biological activity studied in this species until now, with the exception of one study of the antimicrobial activity of certain essential oils. This study focused primarily on the determination of antioxidant, antimicrobial, and enzyme-inhibition activity of aqueous ethanol extract of Turkish endemic Achillea cucullata by in vitro methods. The extract exhibited DPPH radical scavenging activity with an IC50 value of 132.55 ± 0.026 μg/mL, the total phenol content was 53.807 ± 0.059 (mg GAE/g), and the total flavonoid content was 21.372 ± 0.026 (mg QE/g), on the dry-weight basis. Antimicrobial activity was evaluated by a micro-dilution method focused on five microorganisms; two Gram-positive [Staphylococcus aureus (ATCC 29213) and Enterococcus faecalis (ATCC 29212)], two Gram-negative [Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 25922)], and one fungal strain [Candida albicans (ATCC 10231)]. Results show that the MIC value for the tested microorganism was higher than 5 mg/mL. In this work, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α-glucosidase enzymes were strongly inhibited by the A. cucullata extract, and the IC50 values for these enzymes were 2.4 μg/mL, 0.26 μg/mL, and 24.75 μg/mL, respectively. Certain acetylcholinesterase inhibitors have been used for treatment of Alzheimer's disease in the past. α-Glucosidase inhibitors are strong drug candidates, as well as potential functional food agents, for deferring the postprandial absorbency of glucose.
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Metabolite characterization, antioxidant, anti-proliferative and enzyme inhibitory activities of Lophira lanceolata Tiegh. ex Keay extracts
Scopus
Havuzumuzda 9 atıf almış
Lophira lanceolata Tiegh. ex Keay, a native tropical plant to west and central Africa, is a multifunctional plant used as timber and also exploited in traditional medicine in Africa. The present study present the pharmacological properties of Lophira lanceolata leaf and stem bark extracts prepared from different extraction procedures (infusion, homogenizer‐assisted extraction, maceration, soxhlet). The detailed phytochemical composition, in vitro antioxidant and antiproliferative assays, as well as the key enzyme (cholinesterases, tyrosinase, α‐amylase and α‐glucosidase) inhibitory potentials of the extracts were evaluated. Chemical profiling confirmed the presence of lanceolatins and lophirones in all analyzed plant parts. Higher levels of total phenolics (156.42 mg gallic acid equivalent (GAE)/g for maceration-water), phenolic acids (165.84 mg caffeic acid equivalent (CE)/g for maceration-water) and flavanols (101.51 mg catechin equivalent (CAE)/g for soxhlet-methanol (MeOH)) were observed in the stem bark extracts. Antioxidant assays showed remarkable free radical scavenging and reducing power activities for all extracts, among which stem bark showed the highest potential. Stem bark extracts (IC50 values: 57.55–93.10 μg/ml for colorectal adenocarcinoma (HT29) and 78.18–89.58 μg/ml for metastatic breast cancer cell line (MCF7)) also showed better antiproliferative effect than leaf (424.14–790.27 μg/ml for HT29 and 374.46–943.09 μg/ml for MCF7) on carcinoma cells while they induced proliferation in normal human cell lines (normal skin fibroblasts (HFF)). In the enzyme inhibitory assays, the methanol extracts of both plant parts were confirmed as effective against AChE, BChE (only stem barks extracts) and α-glucosidase enzymes. Regarding anti-tyrosinase effects, the methanol leaf extracts (122.21–131.17 mg kojic acid equivalent (KAE)/g) and all stem bark extracts (94.58–153.21 mg KAE/g) exhibited inhibitory effects. Findings amassed herein tend to validate the traditional uses of L. lanceolata and advocate for the development of phyto-medicaments based on its extracts.
Atıf Yapan Makale Bilgileri
Kurumlar (6)
Chitkara University, Punjab
Rajpura, India
Department of Biotechnology, University of Rijeka
Rijeka, Croatia
Duy Tan University
Da Nang, Viet Nam
Selçuk Üniversitesi
Selçuklu, Turkey
Université Félix Houphouët-Boigny
Abidjan, Cote d'Ivoire
University of Mauritius
Reduit, Mauritius