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Cytotoxic and enzyme inhibitory potential of two potentilla species (P. speciosa L. and P. reptans Willd.) and their chemical composition
Scopus
Open Access Toplam 345 atıf DOI
In this work, the biological and chemical fingerprints of three extracts (ethyl acetate, methanol, and water) from two Potentilla species (Potentilla reptans and P. speciosa) were investigated. Antioxidant, enzyme inhibitory, and cytotoxic activities were performed for the biological fingerprint. For the chemical characterization, total bioactive components, and individual phenolic components were determined using photometric and HPLC methods, respectively. The main identified phenolic compounds in these extracts were rutin and catechin. Methanol and water extracts contained the highest total phenolic and flavonoid content. The results of antioxidant assays showed that methanol and water extracts displayed higher antioxidant activity compared to the ethyl acetate extract. Generally, methanol and water extracts exhibited higher biological activities correlated with higher levels the bioactive components. For P. speciosa, the methanol extract exhibited the highest enzyme inhibitory activity (except BChE inhibitory activity). P. reptans exhibited also high antiproliferative activity against MCF-7 cells whilst P. speciosa had weak to moderate activity against both of A549 and MCF-7 cell lines. The results suggest that Potentilla species could be potential candidates for developing new phyto-pharmaceuticals and functional ingredients.
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Metabolomics-guided discrimination of constituents in five commercial apple (Malus × domestica Borkh.) cultivars: Assessment of their antioxidant, neuroprotective, and antidiabetic activities for potential use as functional food
Scopus
Havuzumuzda 8 atıf almış
Apples (Malus × domestica Borkh.) are available in different cultivars, we investigated the similarities and differences in the chemical composition and biological activities of the extracted apple oils by hydrodistillation and hexane extraction. The aroma constituents obtained by hydrodistillation and n-hexane extraction of five commercial Egyptian apple cultivars namely Pink Lady (Cripps pink), Red Delicious, Ambrosia, Golden Delicious, and Granny Smith were investigated by Gas chromatography-mass spectrometry (GC-MS). The total phenolic (TPC) and total flavonoid contents (TFC), in vitro antioxidant analysis, and enzyme inhibition assays were performed. Results showed that TPC and TFC were the highest in the hexane extract of pink ladies with values of 12.42 (mg GAE/g FW) and 1.35 (mg RE/g FW), respectively. Similarly, pink lady hexane extract showed the highest efficacy in antioxidant assays like 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), Cupric reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), metal chelation, and phosphomolybdenum tests with values of 25.21 mg TE/g, 39.82 mg TE/g, 19.27 mg TE/g, 28.16 mg EDTAE/g, and 1.36 mmol TE/g, respectively. Assessments of enzyme inhibition potential of the hexane extracts of the five cultivars on acetylcholinesterase (AChE) showed efficacy for ambrosia only with inhibition value at 2.53 mg GALAE/g. All other cultivars showed comparable efficacies in inhibiting tyrosinase, amylase, and glucosidase enzymes. Significant differences were observed in the chemical composition of the five apple cultivars which were reflected by differences in their antioxidant and enzyme inhibition capacities. Results of biological studies were further confirmed with in silico investigations.
Atıf Yapan Makale Bilgileri
Kurumlar (4)
Ain Shams University
Cairo, Egypt
Faculty of Pharmacy, Ain Shams University
Cairo, Egypt
Selçuk Üniversitesi
Selçuklu, Turkey
University of G. d'Annunzio Chieti and Pescara
Chieti, Italy