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Cytotoxic and enzyme inhibitory potential of two potentilla species (P. speciosa L. and P. reptans Willd.) and their chemical composition
Scopus
Open Access Toplam 345 atıf DOI
In this work, the biological and chemical fingerprints of three extracts (ethyl acetate, methanol, and water) from two Potentilla species (Potentilla reptans and P. speciosa) were investigated. Antioxidant, enzyme inhibitory, and cytotoxic activities were performed for the biological fingerprint. For the chemical characterization, total bioactive components, and individual phenolic components were determined using photometric and HPLC methods, respectively. The main identified phenolic compounds in these extracts were rutin and catechin. Methanol and water extracts contained the highest total phenolic and flavonoid content. The results of antioxidant assays showed that methanol and water extracts displayed higher antioxidant activity compared to the ethyl acetate extract. Generally, methanol and water extracts exhibited higher biological activities correlated with higher levels the bioactive components. For P. speciosa, the methanol extract exhibited the highest enzyme inhibitory activity (except BChE inhibitory activity). P. reptans exhibited also high antiproliferative activity against MCF-7 cells whilst P. speciosa had weak to moderate activity against both of A549 and MCF-7 cell lines. The results suggest that Potentilla species could be potential candidates for developing new phyto-pharmaceuticals and functional ingredients.
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Atıf Yapan Yayın
Optimization of ultrasound-assisted extraction from Olea europaea leaves and analysis of their antioxidant and enzyme inhibition activities
Scopus
Havuzumuzda 5 atıf almış
Olive leaves (Olea europaea L.) are rich source of bioactive compounds but are usually declared as bio-waste. Knowledge from traditional medicine, supported by modern research, has shown the high value of this bio-waste, and the great benefits of its use. The first step in exploiting the biological potential of olive leaves is the rationalization of the extraction process, through which extracts with high biological and market value could be obtained. This study aimed to investigate the influence of different extraction parameters, including solvent concentration, time, temperature, and ratio, on total phenolics, total flavonoids, antioxidant and enzyme-inhibiton activities. The optimal extraction conditions were determined using an Artificial Neural Network (ANN) model. The results revealed that the optimal conditions for the extraction of total bioactive compounds and antioxidant activities from olive leaves were obtained using ethanol concentration of 90%, extraction time 15 min, temperature of 45 °C, and plant:ethanol ratio 1:30. Under these conditions, the extract exhibited high levels of total phenolic content (TPC: 89.05 ± 3.9 mg GAE/g), total flavonoid content (TFC: 19.98 ± 0.38 mg RE/g), and potent antioxidant activity (DPPH: 195.12 ± 6.85 mg TE/g, ABTS: 270.73 ± 1.00 mg TE/g, CUPRAC: 66.14 ± 25.27 mg TE/g, FRAP: 233.58 ± 21.33 mg TE/g). Additionally, the extract demonstrated significant inhibitory effects on α-amylase (0.35 ± 0.00 mmol ACE/g) and tyrosinase (49.23 ± 1.22 mg KAE/g) enzymes. Taken together, the presented results may be valuable for the preparation of health-promoting formulations using olive leaves in the pharmaceutical and cosmeceutical industries.
Atıf Yapan Makale Bilgileri
Kurumlar (3)
Erciyes Üniversitesi
Kayseri, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey
University of Novi Sad
Novi Sad, Serbia