Kurumun Atıf Alan Makalesi
Atıf Alan Yayın
Cytotoxic and enzyme inhibitory potential of two potentilla species (P. speciosa L. and P. reptans Willd.) and their chemical composition
Scopus
Open Access Toplam 345 atıf DOI
In this work, the biological and chemical fingerprints of three extracts (ethyl acetate, methanol, and water) from two Potentilla species (Potentilla reptans and P. speciosa) were investigated. Antioxidant, enzyme inhibitory, and cytotoxic activities were performed for the biological fingerprint. For the chemical characterization, total bioactive components, and individual phenolic components were determined using photometric and HPLC methods, respectively. The main identified phenolic compounds in these extracts were rutin and catechin. Methanol and water extracts contained the highest total phenolic and flavonoid content. The results of antioxidant assays showed that methanol and water extracts displayed higher antioxidant activity compared to the ethyl acetate extract. Generally, methanol and water extracts exhibited higher biological activities correlated with higher levels the bioactive components. For P. speciosa, the methanol extract exhibited the highest enzyme inhibitory activity (except BChE inhibitory activity). P. reptans exhibited also high antiproliferative activity against MCF-7 cells whilst P. speciosa had weak to moderate activity against both of A549 and MCF-7 cell lines. The results suggest that Potentilla species could be potential candidates for developing new phyto-pharmaceuticals and functional ingredients.
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Atıf Yapan Yayın
HPLC-MS/MS Profiling and Evaluation of Antioxidant and Enzyme Inhibitory Activities of Glycyrrhiza glabra L. Crude Extracts
Scopus
Havuzumuzda
The present study aimed to investigate the phytochemical and biological screening of Glycyrrhiza glabra L. aerial parts and roots. Extracts were obtained by maceration using solvents of increasing polarity (chloroform, ethyl acetate, and methanol). HPLC-MS/MS analysis led to the identification of 26 phenolic compounds, with hyperoside followed by delphinidin-3,5-diglucoside and isoquercitrin identified as the main constituents of the aerial parts methanolic extract. The total phenolic and flavonoid contents varied in the range of 54.39–144.09 mg GAE/g and 16.47–65.19 mg RE/g, respectively. The phosphomolybdenum assay yielded a total antioxidant capacity of 1.64–3.40 mmol TE/g. The ability of the extracts to neutralize free radicals ranged between 45.27 and 171.00 mg TE/g for DPPH and 94.51 to 454.14 mg TE/g for ABTS assays. Furthermore, the reducing power assays, evinced that the extracts display significant activity with values ranging from 58.48 to 291.61 mg TE/g (FRAP) and 110.12 to 472.24 mg TE/g (CUPRAC). Moreover, G. glabra L. exhibited notable inhibitory effects against several enzymes (acetylcholinesterase, butyrylcholinesterase, α-tyrosinase, α-glucosidase, and α-amylase). Overall, the obtained results provide scientific evidence supporting the traditional use of the plant and suggest its potential as a promising source of bioactive compounds for further pharmacological investigation.
Atıf Yapan Makale Bilgileri
Kurumlar (5)
Al-Muthanna University
Samawah, Iraq
Faculté des Sciences de Monastir
Monastir, Tunisia
Selçuk Üniversitesi
Selçuklu, Turkey
Technical University of Denmark
Lyngby, Denmark
Università degli Studi di Camerino
Camerino, Italy