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In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
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Exploring bioactive components of sugar bean with some data mining algorithms: relationships between antioxidants, enzyme inhibitors, and minerals
Scopus
Havuzumuzda Open Access
Sugar bean (Phaseolus vulgaris L.) is a legume of notable nutritional and functional value, and its genotypes have substantial variation in bioactive compounds, antioxidant activity, mineral composition, and enzyme inhibition properties. This study aimed to comprehensively evaluate the biochemical and functional diversity of 10 sugar bean genotypes using data mining algorithms to identify superior candidates for functional food and nutraceutical development. Associations between the phytochemical profiles of sugar bean genotypes and their bioactivity, antioxidant capacity, and enzyme inhibition were modeled with classification and regression trees (CART), random forests (RF), and multivariate adaptive regression splines (MARS), while one-way ANOVA quantified varietal effects on these responses. Data mining algorithms, particularly RF and MARS, provided accurate and robust predictions for the relationships between biological, antioxidant, and phytochemical properties of genotypes; however, the predictive power for enzyme inhibition was limited. Statistically significant differences (p < 0.01) were observed among genotypes in extract yield (5.92–7.92%), protein ratio (23.92– 28.70%), total phenolic content (up to 12.28 mg GAE/g), and flavonoid content (up to 0.48 mg QE/g). The Mor Toparlak genotype had superior total phenolic content, antioxidant activity, and acetylcholinesterase inhibitory effects. The Mor Toparlak, Asmaçam, Akçabelen, and Beyşehir genotypes showed the highest α-amylase and α-glucosidase inhibitory activities. The findings showed the significant diversity in nutritional and functional properties among sugar bean genotypes and emphasized which phytochemical traits and metabolic factors can be associated with biological activity, antioxidant activity, and enzyme inhibition. Sugar bean genotypes with high antioxidant capacity, rich mineral content, significant protein levels, and notable enzyme inhibition potential are of great importance for human nutrition and health. In particular, the Mor Toparlak, Kozak, Asmaçam, Akçabelen, and Beyşehir genotypes stand out as valuable raw materials for the development of functional foods and breeding programs aimed at enhancing health benefits, as well as for the creation of health-promoting, palatable, and high-quality genotypes.