Kurumun Atıf Alan Makalesi
Atıf Alan Yayın
In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
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Atıf Yapan Yayın
GC/MS analysis, in vitro antioxidant and enzyme inhibitory activities of the n-hexane extract of Gmelina philippensis CHAM and in silico molecular docking of its major bioactive compounds
Scopus
Havuzumuzda 5 atıf almış
Gmelina philippensis is a decorative plant distributed in South Asia and the Mediterranean region. The n-hexane extract from G. philippensis leaves has been investigated for its chemical composition, antioxidant activity, and enzyme inhibition against six enzymes – acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, amylase, and glucosidase. The main components were fatty acids, esters, and hydrocarbons, with glycidyl palmitoleate (22.11%) and 2-methyl hexacosane (10.75%) dominating in the GC/MS analysis. The extract exhibited higher Cu + + and Fe + + + reducing capacity and chelating with values of 60.18 ± 0.58 mg TE/g, 31.48 ± 0.72 mg TE/g, and 31.11 ± 0.52 mg EDTAE/g. It also displays anti-DPPH (13.46 mg TE/g) and anti-ABTS (23.50 mg TE/g) activities. Enzyme inhibition tests showed that the n-hexane extract has the best anti-tyrosinase activity (39.97 mg KAE/g) and recorded high anti-BChE activity (3.54 mg GALAE/g). The α-amylase and α-glucosidase inhibitory activity of the n-hexane extract recorded values of 0.57 and 1.62 mmol ACAE/g. In addition, the in-silico study demonstrated that the major compounds had satisfactory binding scores when docked with the enzymes under study. Hence, the n-hexane extract of G. philippensis shows great potential as a candidate for the advancement of new therapeutic approaches for oxidative stress, neurodegeneration, and diabetes mellitus.
Atıf Yapan Makale Bilgileri
Kurumlar (5)
Ain Shams University
Cairo, Egypt
Faculty of Pharmacy, Ain Shams University
Cairo, Egypt
Field of Pharmacy
Ras Sedr, Egypt
School of Pharmacy
Badr, Egypt
Selçuk Üniversitesi
Selçuklu, Turkey