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In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
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Mapping the Chemodiversity, Antioxidant and Enzyme Inhibitory Potential and In Silico Studies of Heliotropium europaeum
Scopus
Havuzumuzda Open Access 3 atıf almış
The annual plant Heliotropium europaeum grows in waste ground next to agricultural land and along roadsides from June to September. This plant is indigenous to Southern and Central Europe, as well as Western Asia and Northern Africa; the term “Europaeum” most likely refers to the continent of Europe. According to a literature search, H. europaeum is used externally to cure warts and to aid in wound healing. It also has antibacterial, antifungal, antitumor, anti-inflammatory, insecticidal, antispasmodic, cholagogue, emmenagogue, antipyretic, and anthelmintic properties. In the present investigation, we measured the biological activities of the extracts of H. europaeum, identification of secondary metabolites by UHPLC–MS/MS, and bioinformatics like docking studies against enzymes as evidence for the bioactivities. The results of chemical profiling showed that the HE-M fraction had the highest phenolic and flavonoid contents (15.63 ± 0.39 mg GAE/g extract and 5.67 ± 0.03 mg QE/g extract, respectively), while the HE-E fraction came in second (13.73 ± 0.35 mg GAE/g extract and 4.38 ± 0.08 mg QE/g extract, respectively). Similarly, out of all the extracts, the methanolic extract was found to have the highest antioxidant activity in DPPH and ABTS antioxidant assays, while the HE-E extract was found active among all the extracts in all other antioxidant assays. In the enzyme inhibition assay, we had mixed results, where the HE-E fraction was active against all the enzymes. The chemical profiling through UHPLC–MS/MS analysis endorsed the presence of many known pyrrolizidine alkaloids, flavonoids, and phenolic compounds, while the results of docking studies against the tested enzyme revealed the inhibitory action of pyrrolizidine alkaloids, flavonoids, and phenolic compounds. Further multivariate analysis was employed to find the relationship between total bioactive components and biological activities. This study established a foundation for utilizing these results and compounds as drug leads and for exploring the industrial applications of H. europaeum in developing products aimed at non-communicable diseases.
Atıf Yapan Makale Bilgileri
Kurumlar (6)
King Khalid University
Abha, Saudi Arabia
Selçuk Üniversitesi
Selçuklu, Turkey
The Government Sadiq College Women University Bahawalpur
Bahawalpur, Pakistan
The Islamia University of Bahawalpur
Bahawalpur, Pakistan
University of Education
Lahore, Pakistan
University of Karachi
Karachi, Pakistan