Kurumun Atıf Alan Makalesi
Atıf Alan Yayın
In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
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Atıf Yapan Yayın
Helianthemum oelandicum subsp. incanum and Fumana thymifolia: Characterization of LC-ESI-QTOF-MS profiles and their biological activities based on plant parts and extraction solvents
Scopus
Havuzumuzda Open Access 5 atıf almış
The members of the genera Helianthemum and Fumana offer great potential for ethnobotanical purposes, including gastrointestinal and reproductive problems. In the current study, we focused on the chemical profile and biological properties of Helianthemum oelandicum subsp. incanum and Fumana thymifolia extracts. The extracts from the aerial parts and roots of these plants, which were obtained using n-hexane, ethyl acetate, methanol, and water, were examined. Additionally, we determined the chemical composition of the extracts through liquid chromatography-electrospray ionization-quadrupole time-of-flight-mass spectrometry (LC-ESI-QTOF-MS) and assessed their antioxidant activity using different assays. Moreover, the study involved the evaluation of acetylcholinesterase (AChE), butrycholinesterase (BChE), tyrosinase, amylase, and glucosidase activities in all the extracts. In general, benzoic acid derivatives were the major phenolic acids in both plants. Regarding antioxidant properties. In terms of H. oelandicum subsp. incanum, the aerial part methanol extract displayed the highest TPC, as a result of the highest free radical scavenging activity (DPPH: 628.93 mg TE/g and ABTS: 910.56 mg TE/g). Concerning the inhibitory effects, among H. oelandicum subsp. incanum extracts, the ethyl acetate extract of aerial parts demonstrated the highest AChE inhibition (2.44 mg GALAE/g), with the highest value found in the n-hexane extract of the roots recorded at 2.74 mg GALAE/g. In terms of tyrosinase inhibition, the methanol extracts were more active than other extracts for both plants and their parts. Taken together, these results suggest that H. oelandicum subsp. incanum and F. thymifolia may be useful for potential applications in neuroprotection, skin, and diabetes.
Atıf Yapan Makale Bilgileri
Kurumlar (3)
Ege Üniversitesi
Izmir, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey
University of G. d'Annunzio Chieti and Pescara
Chieti, Italy