Kurumun Atıf Alan Makalesi
Atıf Alan Yayın
In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
Atıf Kaynağı
Atıf Yapan Yayın
Decoding Chemical Profiles, Biological Functions, and Medicinal Properties of Liquidambar orientalis Extracts Through Molecular Modeling and Bioinformatic Methods
Scopus
Havuzumuzda Open Access 14 atıf almış
Liquidambar orientalis, the Anatolian sweetgum tree, is a relict and endemic species in Southwestern Turkey, traditionally used for therapeutic purposes. Our study comprehensively evaluated the therapeutic potential of L. orientalis extracts from its aerial parts to maximize bioactive compound extraction using methanol, ethyl acetate, and water as solvents. The methanolic extract exhibited the highest phenolic (73.04 ± 3.94 mg gallic acid equivalent [GAE]/g) and flavonoid content (48.86 ± 0.76 mg rutin equivalent [RE]/g), demonstrating superior antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH) (256.61 ± 1.70 mg Trolox equivalent [TE]/g), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (308.41 ± 3.14 mg TE/g), and cupric ion–reducing antioxidant capacity (CUPRAC) (411.13 ± 8.48 mg TE/g) assays. It also showed significant enzyme inhibition for acetylcholinesterase (4.43 ± 0.09 mg galanthamine equivalent [GALAE]/g), tyrosinase (149.16 ± 1.14 mg kojic acid equivalent [KAE]/g), amylase (0.93 ± 0.02 mmol acarbose equivalent [ACAE]/g), and glucosidase (1.60 ± 0.01 mmol ACAE/g), suggesting potential applications in neurodegenerative disease management, skincare, and diabetes treatment. Furthermore, methanol and water extracts displayed promising antimicrobial activity due to phenolic compounds such as chlorogenic acid and methyl-3-O-caffeoyl quinate. The methanolic extract exhibited potent anticancer effects against lung cancer (A549) cells, with significant reductions in cell viability and induction of autophagy. The aqueous extract showed remarkable efficacy against prostate cancer (PC3) cells, modulating apoptosis markers. Breast cancer cells (MDA-MB-231) exhibited differential responses, with ethyl acetate extract promoting apoptosis and water extract–enhancing autophagy. Furthermore, molecular docking and dynamics simulations provided additional evidence supporting the therapeutic potential of key phytochemicals from L. orientalis, particularly afzelin and epigallocatechin, against cancer-related targets and bacterial enzymes. Overall, this study fills a gap in understanding the enzyme inhibitory and diverse anticancer effects of L. orientalis extracts, highlighting its potential multi-targeted therapeutic applications, particularly in cancer therapy.
Atıf Yapan Makale Bilgileri
Kurumlar (8)
Cetiz Lab. Sanlıurfa
Sanliurfa, Turkey
Hamad Bin Khalifa University, College of Health and Life Sciences
Doha, Qatar
Kastamonu University
Kastamonu, Turkey
Sabancı Üniversitesi
Tuzla, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey
The Aga Khan University
Karachi, Pakistan
University of G. d'Annunzio Chieti and Pescara
Chieti, Italy
University of Nyíregyháza
Nyíregyháza, Hungary