Kurumun Atıf Alan Makalesi
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In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
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Atıf Yapan Yayın
Comparative metabolic profiling of Cordia myxa leaves and fruits based on UPLC-ESI/MS-MS analysis and investigation of antioxidant activities and enzyme inhibitory properties
Scopus
Havuzumuzda Open Access 10 atıf almış
Cordia myxa, Assyrian plum, is an edible deciduous plant belonging to the family Boraginaceae and exhibited different traditional and pharmacological activities, including wound healing, emollient, expectorant, hepatoprotective, immune-modulator, and hypoglycemic. Our study aimed to investigate the metabolic profiling of C. myxa leaves and fruits growing in Egypt using the Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MSn) technique. Alongside, we determined its antioxidant properties and enzyme inhibitory activity against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, α-glucosidase, and α-amylase. UPLC-MSn analysis revealed the identification of 24 and 19 compounds in the leaf and fruit extracts, respectively. The identified compounds cover different phytochemical classes like phenolic acids, flavonoids, phenolic glycosides, lignans, anthocyanins and fatty acids, flavonoids being the predominant one. The leaf extract showed stronger antioxidant properties (DPPH: 100.80 mg TE/g; ABTS: 134.43 mg TE/g, CUPRAC: 290.87 mg TE/g; FRAP: 205.78 mg TE/g), compared to the fruit extract (DPPH: 22.45 mg TE/g; ABTS: 91.74 mg TE/g; CUPRAC: 41.39 mgTE/g; FRAP: 33.63 mg TE/g). In contrast to other antioxidant assays, the metal-chelating ability of the fruit extract (20.59 mg EDTAE/g) was greater than that of the leaf extract (9.94 mg EDTAE/g). The AChE inhibitory effect of fruit extract (2.59 mg GALAE/g) was higher than that of leaf extract (2.51 mg GALAE/g), but the leaf extract (1.68 mg GALAE/g) showed a stronger BChE inhibitory effect than the fruit extract (1.25 mg GALAE/g). Further, the fruit extract showed almost 2.5 times greater tyrosinase inhibitory activity than the leaf extract. The α-amylase and α-glucosidase inhibitory activities of the leaf extract were superior to those of the fruit extract. The molecular docking studies showed high docking affinities between three compounds, quercetin, kaempferol, and caffeic acid, with all targeted enzymes. As a conclusion, C. myxa signifies an excellent natural supply of treasured bioactive constituents with promising antioxidant and enzyme inhibitory effects. Further phytochemical studies should be performed to isolate the responsible compounds and investigate their mechanism of action.
Atıf Yapan Makale Bilgileri
Kurumlar (2)
Faculty of Pharmacy, Ain Shams University
Cairo, Egypt
Selçuk Üniversitesi
Selçuklu, Turkey