Kurumun Atıf Alan Makalesi
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In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
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UHPLC-MS/MS-guided profiling and bioactivity screening of Papaver apokrinomenon extracts: Insights into alkaloid- and phenolic-driven health effects
Scopus
Havuzumuzda 2 atıf almış
Papaver species are widely known for their remarkable ability to produce a diverse array of secondary metabolites, notably alkaloids. Among them, P. apokrinomenon stands out as a significant species, yet its chemical profile and biological activities remain unexplored. To address this, extracts using ethyl acetate, ethanol, and a 70/30 ethanol-water mixture were generated through methods such as Homogenizer-assisted extraction (HAE), Maceration (MAC), and Ultrasound-assisted extraction (UAE). Various in vitro assays, namely DPPH, ABTS, FRAP, CUPRAC, PBD, and metal chelation tests, were utilized for assessing the antioxidant capabilities of these extracts. Furthermore, the extracts of P. apokrinomenon were analyzed for their inhibitory effects on enzymes like cholinesterases (AChE, BChE), tyrosinase, and those involved in diabetes (α-amylase and α-glucosidase). UHPLC-MS/MS analysis detected several alkaloids, including salsolinol, salutaridinol, norcoclaurine, and salutaridine, within the extracts. Findings indicated that the extracts from both 70 % ethanol and ethanol (extracted via MAC and UAE) demonstrated superior radical scavenging, reduction potential, and overall antioxidant function. Moreover, all extraction methods yielded 70 % ethanol and ethanol extracts that showed strong enzyme inhibitory activities across the board. Supporting in silico analyses, such as structure-based docking against AChE, BChE, α-amylase, and α-glucosidase, complemented by 100 ns MD simulations and MM/GBSA calculations, indicated favorable binding energies for pivotal alkaloids against each enzyme, aligning qualitatively with the in vitro enzyme inhibition. These findings propose that P. apokrinomenon could be promising for traditional medicine use and hold potential for development into natural health products, targeting various pharmaceutical-related therapies.
Atıf Yapan Makale Bilgileri
Kurumlar (3)
Harran Üniversitesi
Sanliurfa, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey
University of Nyíregyháza
Nyíregyháza, Hungary