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In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
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Exploration of the Chemical Components and Biological Activities of Hippophae salicifolia Extracts: In Vitro, Molecular Docking, and MD Simulation Approaches
Scopus
Havuzumuzda Open Access 3 atıf almış
Hippophae salicifolia, commonly known as sea buckthorn, is native to the Indian Himalayan region. This study is the first to comprehensively assess the phytochemical profile and biological activities of H. salicifolia leaves extracted through maceration, infusion, and percolation (Soxhlet apparatus) methods. The extracts were analyzed for total flavonoid and phenolic content. Percolation extract exhibits the highest flavonoid content (35.66 ± 0.81 mg RE/g) and the infusion extract shows the highest phenolic content (163.55 ± 1.49 mg GAE/g). UHPLC–MS/MS profiling identified 57 phytoconstituents, including phenolic compounds, alkaloids, fatty acids, and miscellaneous metabolites. Antioxidant activities were assessed using DPPH, ABTS, CUPRAC, FRAP, PBD, and MCA assays, with maceration extracts demonstrating superior activity in most assays. Enzyme inhibitory studies revealed significant AChE, BChE, tyrosinase, α-amylase, and α-glucosidase inhibition by maceration and percolation extracts. In silico molecular docking and dynamics studies provided insights into the stable interactions of bioactive compounds, such as quercetin, myricetin, kaempferol, and linoleic acid, with target proteins (AChE, amylase, BChE, glucosidase, and tyrosinase). These results underline the therapeutic potential of H. salicifolia leaves for functional food and the nutritional development with effects on improving the socioeconomic conditions of the Himalaya communities.
Atıf Yapan Makale Bilgileri
Kurumlar (5)
Delhi Pharmaceutical Sciences and Research University
New Delhi, India
Edvancer - a part of Global University System
Mumbai, India
GNA University
Phagwara, India
Selçuk Üniversitesi
Selçuklu, Turkey
University of Nyíregyháza
Nyíregyháza, Hungary