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In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
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Diplotaxis tenuifolia (L.) DC.: phytochemical profiling and multifunctional biological evaluation of extracts obtained by maceration and ultrasound-assisted extraction
Scopus
Havuzumuzda Open Access
Diplotaxis tenuifolia is an edible Brassicaceae species recognized for its phytochemical richness and health-promoting potential. This study evaluated six extracts obtained by maceration (MAC) and ultrasound-assisted extraction (UAE) using ethyl acetate (EtOAc), methanol (MeOH), and water. LC-MS/MS profiling revealed solvent- and method-dependent metabolite occurrence, including glucosinolates, flavonol glycosides, phenolic acids, and long-chain fatty acids, with representative compounds such as glucoraphanin, quercetin- and kaempferol-derived glycosides, p-coumaric and ferulic acids, and α-linolenic, linoleic, and erucic acids. UAE enhanced phytochemical diversity, particularly in water extracts, increasing detected compounds from 16 to 30. Total phenolic and flavonoid contents ranged from 15.80 to 24.07 mg GAE per g and from 2.06 to 31.53 mg RE per g, respectively. In antioxidant assays, MeOH extracts showed the highest DPPH activity (8.55 mg TE per g), whereas EtOAc-UAE exhibited the strongest CUPRAC and metal-chelating activities (45.67 mg TE per g and 22.14 mg EDTAE per g). In enzyme inhibition assays, MeOH-UAE showed the highest acetylcholinesterase inhibition (1.99 mg GALAE per g), while EtOAc-UAE was most active against butyrylcholinesterase, α-amylase, and α-glucosidase (3.97 mg GALAE per g, 0.89 mmol ACAE per g, and 0.64 mmol ACAE per g). EtOAc extracts were the only fractions active against Bacillus subtilis (6.7 and 8.7 mm for EtOAc-MAC and EtOAc-UAE), while EtOAc-UAE reduced Staphylococcus aureus biofilm formation to 13.6% of the control. EtOAc-UAE also showed the strongest cytotoxicity in MDA-MB-231 and PC-3 cells (IC50: 306.3 and 389.6 µg mL−1), with RT-qPCR confirming approximately 2-fold bax and 3-fold LC3B upregulation. These findings demonstrate that solvent polarity and extraction method critically determine the phytochemical and biological profiles of D. tenuifolia extracts.
Atıf Yapan Makale Bilgileri
Kurumlar (5)
Kastamonu University
Kastamonu, Turkey
Marmara Üniversitesi
Istanbul, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey
University of Novi Sad
Novi Sad, Serbia
University of Nyíregyháza
Nyíregyháza, Hungary