Kurumun Atıf Alan Makalesi
Atıf Alan Yayın
In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
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Atıf Yapan Yayın
Exploring the therapeutic potential of Cousinia iconica Hub.-Mor extracts through chemical profiling, in vitro and in-silico assays
Scopus
Havuzumuzda Open Access 3 atıf almış
Some species of the genus Cousinia (family Asteraceae) are traditionally used to treat various diseases, and scientific research has demonstrated that they possess interesting biological activities. This study offers the first comprehensive evaluation of the aerial parts of Cousinia iconica Hub.-Mor, incorporating phytochemical profiling, bioactivity assays, and network pharmacology. High-resolution LC–ESI-QTOF–MS analysis revealed that the plant is rich in phenolic acids, hydroxy- and methoxy-flavonoid glycosides, and lignans. The aqueous extract contained the highest levels of total phenolics (78 mg gallic acid equivalents/g) and flavonoids (15.5 mg rutin equivalents/g). It also exhibited the strongest radical scavenging, reducing power, and metal chelating activities. Biological tests showed that, while all three organic extracts had similar cytotoxic effects against the RKO colorectal cancer cell line, none demonstrated measurable antiviral activity against HHV-1 or human coxsackievirus B3. In silico analyses supported these results by identifying 22 disease-relevant targets of C. iconica compounds linked to head and neck squamous cell carcinoma (HNSCC). Molecular docking showed strong binding affinities of key phytochemicals to cancer-related targets (CDK2, CHEK1, PDGFRA) and metabolic enzymes (acetylcholinesterase, butyrylcholinesterase, tyrosinase, amylase, glucosidase). Overall, the results highlight C. iconica as a promising source of bioactive phenolic compounds with significant cytotoxic potential, though its antiviral effects appear limited. Further research, especially on isolated constituents and mechanism-based assays, is necessary to better understand its therapeutic potential.
Atıf Yapan Makale Bilgileri
Kurumlar (5)
Harran Üniversitesi
Sanliurfa, Turkey
Khartoum University
Khartoum, Sudan
Medical University of Lublin
Lublin, Poland
Selçuk Üniversitesi
Selçuklu, Turkey
Università degli Studi di Padova
Padua, Italy