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In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
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Atıf Yapan Yayın
Multi-faceted bioactivity of three unexplored Turkish plants: A comparative phytochemical and pharmacological profiling
Scopus
Havuzumuzda Open Access 4 atıf almış
Since ancient times, plants have been used as a valuable source for treating serious diseases. Three different plants, namely Aubrieta pinardii , Johrenia dichotoma , and Malabaila dasyantha were used and extracted using the extraction solvents ethyl acetate (EA), methanol (MeOH), and water. Antioxidant activity was determined using several in vitro assays. Subsequently, the enzyme inhibition activities of the extracts were evaluated against cholinesterases (AChE and BChE), tyrosinase, amylase, and glucosidase. The antibacterial and antifungal effects of the extracts were assessed. Methanol extracts of A . pinardii , J . dichotoma , and M . dasyantha yielded the highest total flavonoids (TFC) compared to EA and water extracts. The analysis using HPLC-ESI-Q-TOF-MS identified various classes of phytochemicals within the species examined. Aubrieta pinardii extracts were abundant in glucosinolates, whereas J. dichotoma and M. dasyantha extracts were characterized by the presence of coumarins and furanocoumarins. The EA extracts exhibited greater inhibition of microorganism growth compared with MeOH and water extracts. Notably, J. dichotoma showed similar or superior antifungal activity against A. niger (MIC/MFC 0.25/0.50 mg/mL) and M. dasyantha demonstrated comparable efficacy against T. viride (MIC/MFC 0.50/1.00 mg/mL) and P. verrucosum var. cyclopium (MIC/MFC 0.125/0.25 mg/mL) when benchmarked against the reference drug ketoconazole. These results provide valuable information about A . pinardii , J . dichotoma , and M . dasyantha chemical profiles and underline the importance of selecting appropriate solvents for the extraction of metabolites from plant matrix for potential applications in the treatment of various diseases.
Atıf Yapan Makale Bilgileri
Kurumlar (5)
Renji Hospital
Shanghai, China
Selçuk Üniversitesi
Selçuklu, Turkey
University of Belgrade
Belgrade, Serbia
University of G. d'Annunzio Chieti and Pescara
Chieti, Italy
University of Nyíregyháza
Nyíregyháza, Hungary