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In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
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Experimental and theoretical exploration of biological activities of different extracts of Equisetum arvense: Enzyme inhibition, DFT, molecular docking and molecular dynamics analyses
Scopus
Havuzumuzda 1 atıf almış
In this study, the antioxidant and enzyme inhibition capacities of acetone, ethanol, and water extracts of E. arvense were comprehensively investigated using both experimental and theoretical approaches. The phytochemical constituents of the extracts were characterized by a validated LC-MS/MS method, which identified 53 bioactive molecules commonly found in medicinal plants, including prominent compounds such as Isoquercitrin, Quinic acid, Acacetin, Aconitic acid, Caffeic acid, and Protocatechuic acid. Among the tested extracts, acetone exhibited the highest levels of phenolic and flavonoid contents, and accordingly demonstrated the strongest antioxidant activity as evaluated by DPPH, ABTS, CUPRAC, FRAP, and other assays. Enzyme inhibition studies showed the strongest effect for the ethanol extract in cholinesterase inhibition tests. Similarly, the greatest inhibition of tyrosinase was observed with the ethanol extract. To elucidate the molecular basis of these bioactivities, dominant compounds were subjected to density functional theory (DFT) calculations and molecular docking simulations against key enzymes. Theoretical results confirmed that Isoquercitrin and Acacetin possess favorable electronic and binding properties, suggesting their role in the observed bioactivities. These findings highlight E. arvense as a promising natural source of multifunctional phytochemicals with potential applications in managing oxidative stress and metabolic disorders.
Atıf Yapan Makale Bilgileri
Kurumlar (4)
Cumhuriyet Üniversitesi
Sivas, Turkey
Dicle Üniversitesi
Diyarbakir, Turkey
Ege Üniversitesi
Izmir, Turkey
Selçuk Üniversitesi
Selçuklu, Turkey