Kurumun Atıf Alan Makalesi
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In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
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Atıf Yapan Yayın
Deciphering organ-specific chemistry and bioactivities of Plumeria rubra L. via integrated metabolomics, biological evaluation, and molecular docking studies
Scopus
Havuzumuzda
Despite the extensive ethnomedicinal use of Plumeria rubra L. (Apocynaceae), organ-specific metabolic specialization and its link to pharmacological properties remain poorly understood. Using LC–HRMS/MS combined with GNPS molecular networking, 160 metabolites across 14 phytochemical classes were annotated, revealing clear organ-specific profiles via multivariate analyses (PCA, HCA, OPLS-DA) and KEGG pathway enrichment analyses. Flowers were dominated by flavonoids and phenylpropanoids, leaves by iridoids and triterpenes, and stems by lignans. KEGG pathway enrichment further highlighted differential activation of monoterpenoid, phenylpropanoid, and flavonoid biosynthesis pathways among organs. A panel of in vitro assays demonstrated strong antioxidant activities across all organs, particularly in flowers, whereas enzyme inhibition varied by organ, leaves showed the strongest AChE inhibition, stems exhibited higher BChE and α-amylase inhibition, and both leaves and flowers displayed potent anti-tyrosinase and α-glucosidase activity. Pearson's correlation analysis revealed strong positive relationships between total phenolic and flavonoid contents and antioxidant activities, while enzyme inhibitory properties were primarily associated with iridoid, lignan, and triterpene abundance, highlighting chemistry–bioactivity coherence. Metabolite–bioactivitycorrelation further identified key biomarkers, such as quercetin glycosides, caffeoylquinic acids, plumieride, liriodendrin, and arjunolic acid, as major drivers of the observed pharmacological effects. Molecular docking supported these findings, demonstrating that the iridoid allamancin and the lignan hydroxypinoresinol establish stable interactions with AChE, tyrosinase, and α-glucosidase, showing multi-target inhibitory potential. Overall, the integration of LC–MS-based metabolomics, statistical modeling, and bioactivity correlation elucidates a clear functional specialization among P. rubra organs, highlighting the species as a promising source of multifunctional phytochemicals with combined antioxidant, neuroprotective, dermatological, and antidiabetic relevance.
Atıf Yapan Makale Bilgileri
Kurumlar (6)
Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa
Caparica, Portugal
Faculty of Pharmacy, Cairo University
Cairo, Egypt
Medical University of Lublin
Lublin, Poland
Selçuk Üniversitesi
Selçuklu, Turkey
Thermo Fisher Scientific
Brisbane, Australia
Victor Chang Cardiac Research Institute
Sydney, Australia