Kurumun Atıf Alan Makalesi
Atıf Alan Yayın
In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
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Atıf Yapan Yayın
Comparative Phytochemical Profiling, Antioxidant, and Enzyme Inhibitory Activities of Different Parts of Aspilia africana Extracted by Maceration and Soxhlet Methods
Scopus
Havuzumuzda
Aspilia africana is a popular medicinal plant mainly found in Africa, with a variety of ethnobotanical uses. The purpose of this research is to make a comparative assessment of the phytochemical content, antioxidant activity, and enzyme inhibition activity of different parts of A. africana prepared by two conventional methods. Leaves, flowers, and the bark of A. africana were subjected to maceration and Soxhlet extraction, and detailed chemical analysis of each of the extracts was carried out using HPLC-ESI-Q-TOF-MS. Antioxidant activities of the extracts were estimated using the following methods: DPPH, ABTS, FRAP, CUPRAC, and MCA, while the enzyme inhibitor activity of the extracts was determined on AChE, tyrosinase, α-amylase, and α-glucosidase. Results from HPLC-ESI-Q-TOF-MS indicated the existence of phenolics (chlorogenic acid, caffeic acid, p-coumaric acid), flavonoids (rutin, quercetin-3,4'-di-O-glucoside), coumarins (scopoletin-7-O-glucoside, esculetin (6,7-dihydroxycoumarin)), and terpenes glycosides among the main active compounds of A. africana responsible for these activities. The highest antioxidant activities were obtained from the leaf extract obtained by Soxhlet extraction. Maceration extracts of flowers and leaves provide the maximum inhibition of tyrosinase (60.38±0.18 mg KAE/g). These results show A. africana as a promising source of antioxidant compounds with their potential applications in neurodegenerative disorders, managing diabetes, and cosmetic formulations.
Atıf Yapan Makale Bilgileri
Kurumlar (5)
Peleforo Gon Coulibaly University
Korhogo, Cote d'Ivoire
Selçuk Üniversitesi
Selçuklu, Turkey
Université d'Abobo-Adjamé
Abidjan, Cote d'Ivoire
University of Novi Sad
Novi Sad, Serbia
University of Nyíregyháza
Nyíregyháza, Hungary