Kurumun Atıf Alan Makalesi
Atıf Alan Yayın
In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca
Scopus
Toplam 432 atıf DOI
The genus Potentilla is interesting for the pharmaceutical field due to its valuable medicinal properties, which have been observed in complementary and alternative medicine. In recent years, studies conducted to estimate the biological activity of several of the Potentilla species have shown a wide spectrum of therapeutic properties. In particular, in the present paper, different extracts obtained from the herb P. thuringiaca were analysed for antioxidant and enzyme inhibitory activities. The UHPLC-DAD-MS3 hyphenated techniques reported herein allow for the identification of phytoconstituents. The analyses showed the presence of flavonoids and ellagitannins as major components. Furthermore, the data demonstrated that the analysed extracts revealed a high total antioxidant capacity in the phosphomolybdenum assay. The free radical scavenging activity of the extracts was evaluated using DPPH and ABTS assays. The reducing power activity of P. thuringiaca was also determined by FRAP and CUPRAC assays, as well as metal chelating activity. In addition, the total extracts and the different fractions of P. thuringiaca revealed potent inhibitory activities against α-amylase and α-glucosidase, AChE, tyrosinase and lipase. Surprisingly, no activity against BChE was shown. P. thuringiaca could be a valuable natural source of antioxidants with interesting inhibitory actions against the key enzymes involved in several human diseases, and could represent a valid starting point for the development of new treatment and management strategies, including its use as a food supplement.
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Atıf Yapan Yayın
How distillation alters bioactivity: Comparative phytochemical and biological evaluation of three Lamiaceae species (Origanum sipyleum, Thymus sipyleus, and Sideritis phrygia) before and after distillation
Scopus
Havuzumuzda
In the current study chemical profiles and the biological activities of three different plant species (Origanum sipyleum, Thymus sipyleus, and Sideritis phrygia) of the Lamiaceae family native to Turkey were explored considering different approaches leading to extracts with different composition. We adopted for each plant extraction with methanol (Total Methanol), water infusion (W), supercritical CO2 extract (SCO2), essential oil (EO). Furthermore, the residues remaining after hydrodistillation and supercritical CO2 extraction were subsequently extracted with methanol, yielding the Spent HD and Spent SCO2 extracts for each plant species. To evaluate possible bioactivities several assays were performed. Antioxidant capacities were measured with multiple in vitro assays (ABTS, DPPH, FRAP, CUPRAC, PBD, and metal chelating ability (MCA), along with total phenolic content. Enzyme inhibitory activities of the obtained extracts were evaluated against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, amylase, and glucosidase, important enzymes involved in metabolic and degenerative disorders like diabetes, neurodegenerative and skin disorders. Methanol and water extracts generally exhibited the highest total phenolic content, along with the greatest antioxidant capacities. Among the remaining extracts, the spent fractions (especially spent SCO₂) also demonstrated notable antioxidant activity. Beneath them the extracts spent (especially spent SCO2) were the most active. The different extraction procedures lead to different chemical composition with limited extraction of phytoconstituents in the SCO2 and larger presence of phenolics in the other extracts. Salvianolic acid B, rosmarinic acid and kaempferol-hexoside-derivate were the main components in the total methanol extracts of O. sipyleum, T. sipyleus and S. phrygia, respectively. In same order, the main components were alloaromadendrene, valencene and spathulenol in their EOs. The results indicate that the plant materials after HD and SCO2 are still rich in bioactive compounds and they can be used to extract bioactive compounds. The obtained extracts present different potential bioactivities and can be the starting point to design multidirectional health-promoting products for applications in pharmaceutical and nutraceutical areas.
Atıf Yapan Makale Bilgileri
Kurumlar (6)
Balikesir Üniversitesi
Balikesir, Turkey
PARD Engineering and Otomasyon
Konya, Turkey
Peleforo Gon Coulibaly University
Korhogo, Cote d'Ivoire
Selçuk Üniversitesi
Selçuklu, Turkey
Università degli Studi di Padova
Padua, Italy
University of Novi Sad
Novi Sad, Serbia